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DNA&RNA electrophoresis-related reagents and buffer

 50X TAE Buffer (pH8.5)
* The concentration of 2M Tris-acetic acid component, 100mM EDTA
* Preparation of volume 1L
* Preparation Method                                                                                                                       

1. The following reagents were weighed, placed in 1L beaker
Tris                            242.2g
Na2 EDTA • 2H2O     37.2g
2. Add about 800ml of deionized water, stir dissolved, then add 57.1ml of acetic acid, the full mix  3. Add deionized water to 1L solution to volume, the room temperature. 

 
10X TBE Buffer (pH8.3)
* Component concentration 890mM Tris-acetate, 20mM EDTA
* Preparation of volume 1L
* Preparation method
1. The following reagents were weighed, placed in 1L beaker
Tris                           108g; 
Na2EDTA · 2H2O     7.44g
Borate                        55g
2. Add about 800ml of deionized water, stir to dissolve.
3. Add deionized water to 1L solution to volume, the room temperature.


  
10X MOPS Buffer
* Component concentration 200mM MOPS, 20mM NaAc, 10mM EDTA
* Preparation of volume 1L
* Preparation method
1. Weigh 41.8g MOPS, placed in 1L beaker, add about 800ml DEPC treated water, stirring to dissolve.
2. Adjust pH with 1M NaOH to 7.0.
3. Then add the following reagents
1M NaAc (DEPC Treatment) 20ml
0.5M EDTA (pH8.0) (DEPC Treatment) 20ml
4. The solution with DEPC treated water volume to 1L.
5. Impurity with a 0.45μm membrane filter at room temperature away from light. (Note: see the light or high temperature sterilization solution will turn yellow, you can still use, but you can not use black) 

  
Ethidium bromide (10mg/ml)
* The concentration of 10mg/ml ethidium bromide component
* Preparation of volume 100ml
* Preparation method
1. Weigh 1.0g ethidium bromide, added to the 200ml container.
2. Deionized water, 100ml, stir a few hours completely dissolved ethidium bromide.
3. Brown bottle into the solution at room temperature away from light.
4. Ethidium bromide concentration of the final work of 0.5μg/ml.

  
6X DNA Loading Buffer (single-dye)
* Component concentration
0.25% (W / V)     bromophenol blue
30% (V / V)        glycerol
* Preparation of volume  10ml
* Preparation method
1. The following reagents were weighed, placed in 15ml plastic centrifuge tube
25mg    bromophenol blue
2. 6ml to the centrifuge tube with deionized water, stir to dissolve.
3. Mix by adding 3ml glycerol, deionized water final volume to 10ml, room temperature. 

  
6X DNA Loading Buffer (double-dye)
* Component concentration
0.25% (W / V)     bromophenol blue
0.25% (W / V)     Xylene Gyanol FF
30% (V / V)         glycerol
* Preparation of volume 10ml
* Preparation method
1. The following reagents were weighed, placed in 15ml plastic centrifuge tube
25mg bromophenol blue
Blue FF 25mg xylene nitrile
2. 6ml to the centrifuge tube with deionized water, stir to dissolve.
3. Mix by adding 3ml glycerol, deionized water final volume to 10ml, room temperature.

  
10X RNA Loading Buffer (single-dye)
* Component concentration
10mM                    EDTA
0.25% (W / V)       bromophenol blue
50% (V / V)           glycerol
* Preparation of volume 10ml
* Preparation method
1. The following reagents were weighed, placed in 15ml plastic centrifuge tube
0.5M EDTA (pH 8.0) 200μl
25mg bromophenol blue
2. To the centrifuge tube 4ml DEPC treated water, stir to dissolve.
3. Adding 5ml glycerin mixing, constant volume with DEPC treated water to 10ml, room temperature.

  
10X RNA Loading Buffer (RNA electrophoresis only)
* Component concentration
10mM                     EDTA                                                                                                                      

0.25% (W / V)       bromophenol blue
0.25% (W / V)       Xylene Gyanol FF
50% (V / V)           glycerol
* Preparation of volume 10ml
* Preparation method
1. The following reagents were weighed, placed in 15ml plastic centrifuge tube
200μl        0.5M EDTA (pH 8.0)     

25mg        bromophenol Blue                                                                                                  

25mg        Xylene Gyanol FF                                                                                                            

2. Tube 4mlDEPC to deal with water, stir to dissolve.
3. Adding 5ml glycerin mixing, constant volume with DEPC treated water to 10ml, room temperature. 

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