Home About Us News Products Technical Support Download Selling Network Recruitment Feedback Contact Us
Product class
DNA MarkersLadders
Plasmid Isolation
PCR Product/Gel Purification
Genomic DNA Isolation
RNA Isolation
Protein Analysis
Electrophoresis Products
Molecular Biology Reagent
DNA/RNA Spin column
Lab Instruments
Technical Support You are here:Home >> Technical Support
PCR Amplification Kit
Cat # PG011  200 reactions
PCR Amplification Kit is designed to perform PCR reaction on any DNA template and supplies all the reagents necessary for PCR  DNA, a control template accompanied with control primers for amplification of DNA target, and pHY Size Marker are also included to verify and secure kit performance   Storage
Kit components
HS Taq polymerase(5 units/µl)
50 µl
dNTP Mixture (each 2.5 mM)
1.28 ml
10 PCR Buffer
(100 mM Tris-HCl, pH8.3, 500 mM KCl, 15 mM MgCl2)
1 ml
10 PCR Buffer (Mg2+ free)
(100 mM Tris-HCl, pH8.3, 500 mM KCl)
1 ml
MgCl2 (25 mM)
1 ml
Control Template (1 µg/ml DNA)
100 µl
Control Primer 1 (20 pmol/µl)
50 µl
Control Primer 2 (20 pmol/µl)
50 µl
Control Primer 3 (20 pmol/µl)
50 µl
-EcoT14 I digest (100 ng/µl
40 µl
6 Loading Buffer
1 ml
Note: Using Control Template, 6012 bp fragment can be amplified with Control Primers 1 and 2, and 500 bp fragment with Control Primers 1 and 3 

Protocol :
A. Control experiment
This kit includes DNA and primers for target region of DNA (6012bp or 500bp).
(1) Prepare the reaction mixture in a PCR microtube for PCR by combining the following reagents to be the total volume of 50 l.
Final  concentration
10X PCR Buffer
5 l
dNTP Mix(each 2.5 mM)
4 l
each 200 M
Control Primer 1
0.5 l
0.2 M
Control Primer 2 or 3
0.5 l
0.2 M
HS Taq polymerase
0.25 l
1.25 units/50 l
Control  Template 
0.5 l
0.5 ng/50 l
39.25 l
50 l
  Note:10XPCR Buffe(MgFree) and MgCl2 solution shall be used instead
of10XPCR Buffer if necessary.
  (2) If necessary overlay mineral oil.
(3) Place tubes in a thermal cycler.
(4) Perform the reaction under the following conditions.
When amplifying 6012 bp with control primer 1 and 2.
[ 94, 1min (denaturation) 68,4min (annealing and extension) ] 30cycles
72, 5 min, 1cycle
When amplifying 500 bp with control primer 1 and 3.
[ 94, 30 sec(denaturation)55, 30sec(annealing)72, 30sec(extension) ] 30cycles
72, 2min, 1 cycle
B. Experiment with the samples
Protocol for the samples is basically the same as he control experiment described in A.
The parameters of each step (temperature, time) must be optimized for DNA templates. Depending on the size of target, the sequence, and length of primers.
C Electrophoresis
1) Take 5- 10l of PCR amplified samples and.add its 1/6 volume. Of 6X Loading Buffer.
2) Pipet the samples into the agarose gel slots, and run the gel. The condition of Agarose gel vary according to sizes of amplified DNA.
3) After electrophoresis is completed Verify the bands of.amplified DNA nder UV illumination.
Home About Us News Products Technical Support Download Selling Network Recruitment Feedback Contact Us
Guangzhou Geneshun Biotech Ltd ICP08201538
Tel020-23312160 Fax020-87056567