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Taq Plus DNA Polymerase

Supplied with
1ml,  10¡ÁTaq Plus Buffer (Mg2+ Plus)
1ml¡Á2,10¡ÁTaq Plus Buffer (Mg2+ Plus)

Concentration:  5u/µl
Store at ¨C20¡ãC
Taq Plus DNA Polymerase is a special formulation designed for amplify large fragment. The main component is Taq DNA Polymerase, and Pfu DNA Polymerase are added to enhance the efficiency of amplification reaction. Theoretically, Taq Plus produces significantly higher yields of PCR products than ordinary Taq Polymerase, especially for fragments >1kb, and can amplify up to 20kb. Taq Plus also contains a proofreading activity that reduces the error rate of Taq Polymerase. Most of the amplified DNA fragments have a 3´A overhanging. However, a small percentage of the amplified DNA fragments are blunt-ended. Taq Plus is suitable as a direct replacement for ordinary Taq Polymerase in most applications.
High fidelity: with an error frequency of 1.6X10-6 during DNA synthesis.
Higher yield: Taq Plus increases the efficiency of polymerization reaction, resulting in a great percentage of extenuation reaction completion up to 20kb.
Unit Definition:
One unit of the enzyme catalyzes the incorporation of 10nmole of deoxyribonucleotides into a polynucleotide fraction in 30min at 74¡æ.
Storage Buffer:
20mM Tris-HCl, pH 8.0, 100mM KCl, 0.1mM EDTA, 5mM DTT, 50% glycerol, 0.5% NP40 and 0.5% Tween 20.
10X Reaction Buffer (Mg2+ Plus):
500mM KCl, 100mM Tris-HCl (pH 9.0 at 25¡æ) and 1% Triton X-100, 100mM (NH4)2SO4, 15mM MgCl2, PCR enhancer
Long PCR (up to 20 kb), PCR cloning, RT-PCR etc.
Quality Control
No contaminating endonuclease or exonuclease activity detected. Functionally tested in PCR.
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