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Hotstar Taq DNA Polymerase

Supplied with
1.25ml,10¡ÁHotstar Taq Buffer
(Mg2+ Plus)

Store at ¨C20¡ãC
Hot Start Taq DNA Polymerase is a recombinant Taq DNA polymerase which has been chemically modified by the addition of heat-labile blocking groups to its amino acid residues. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme is restored during a short 4-minute incubation at 95¡ãC. The activated enzyme maintains the same functionality as Taq DNA polymerase: catalyzes 5'=>3' synthesis of DNA, has no detectable 3'=>5' proofreading exonuclease activity.The High Taq adds a single 3'-A overhang to each end of the PCR product, which can be applied to T/A cloning.
High yield amplification of complex templates
Short 4 min activation time
High PCR specificity, reduce effects of mispriming and primer-dimer formation
Enhanced PCR sensitivity, amplification of fragment up to 5kb
Convenient room temperature PCR set-up
Generates PCR products with 3¡¯-A overhangs
Unit Definition:
One unit of the enzyme catalyzes the incorporation of 10nmole of deoxyribonucleotides into a polynucleotide fraction in 30min at 74¡æ.
Storage Buffer:
20mM Tris-HCl, pH 8.0, 100mM KCl, 0.1mM EDTA, 5mM DTT, 50% glycerol, 0.5% NP40 and 0.5% Tween 20.
10X Reaction Buffer(with MgCl2):
200mM KCl, 100mM Tris-HCl (pH 9.0 at 25¡æ) and 1% Triton X-100, 50mM (NH4)2SO4, and 15mM MgCl2.
Quality Control
No contaminating endonuclease or exonuclease activity detected.
Functionally tested in PCR.
Hot-start PCR amplification
Specific amplification of complex cDNA and genomic template
Amplification from low copy number DNA template
Real-Time PCR
Multiple PCR
Generation of PCR products for TA cloning
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