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Taq DNA Polymerase(Recombinant)

 

Cat#
Size,Concentration
Supplied with
PR1011
500U(5U/µl)
1.4ml, 10¡ÁPCR Buffer(Mg2+ Plus)
PR1012
500U(5U/µl)
1.4ml, 10¡ÁPCR Buffer(Mg2+ free)
0.5ml, 25mM MgCl2

Store at ¨C20¡ãC
Description:
Taq DNA Polymerase is a highly thermostable DNA polymerase of a thermophilic bacterium Thermus aquaticus. Taq DNA Polymerase catalyzes 5'=>3' synthesis of DNA. The enzyme has no detectable 3'=>5' proofreading exonuclease activity and possesses low 5'=>3' exonuclease activity. This enzyme adds a single 3'-A overhang to each end of the PCR product£¬which can be applied to T/A cloning.
The recombinant Taq DNA Polymerase is ideal for standard PCR of templates 5 kb or shorter.
Storage Buffer
20mM Tris-HCl, 1mM DTT, 0.1mM EDTA,100mM KCl,
50% glycerol, 0.5%NP40, 0.5%TW20
10¡ÁPCR Buffer
750 mM Tris-HCl (pH 8.8 at 25¡ãC), 200 mM KCl,
50 mM (NH4)2SO4,15mM MgCl2, 0.5% NP-40.
Unit definition
One unit is the amount of enzyme required to catalyze the incorporation of 10 nmol of nucleotides into acid insoluble material in 30 min. at 74¡ãC under assay conditions.
Applications
• PCR amplification of DNA fragments up to 5 kb
• DNA labeling and DNA sequencing
• PCR for cloning.
Quality Control
No contaminating endonuclease or exonuclease activity detected.
Functionally tested in PCR.
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