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DNA Markers£¨Ladders£©
Plasmid Isolation
PCR Product/Gel Purification
Genomic DNA Isolation
RNA Isolation
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Electrophoresis Products
Molecular Biology Reagent
DNA/RNA Spin column
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High Purity Plasmid Miniprep Kit(spin column)
Plasmid Miniprep Kit is an innovative system that radically simplifies extraction and purification of nucleic acids from a variety of sources. The Plasmid Miniprep Kit combines the power of silica membrane technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high quality DNA in under half an hour. The silica membrane reversibly, binds DNA or RNA under certain optimal conditions allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with deionized water or low salt buffer. Plasmid Miniprep Kit mini-columns facilitate the binding, washing, and elution steps thus enabling multiple samples to be simultaneously processed. Yields vary according to plasmid copy number, E.coli strain, and conditions of growth, but 1¡«4.5ml of overnight culture in LB medium typically produces 1¡«40µg plasmid DNA. The product is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, and other manipulations.
Kit Content, Storage and Stability

GS1021 (100 preps)
GS1022 (200 preps)
RNase A (10mg/ml)
Buffer P1
25 ml
50 ml
Buffer P2
25 ml
50 ml
Buffer P3
35 ml
70 ml
Buffer PE
31.5 ml
63 ml
Add 18.5ml absolute ethanol before first use
Add 37ml absolute ethanol before first use
Buffer WB
25 ml
50 ml
Add 100ml absolute ethanol before first use
Add 200ml absolute ethanol  before first use
Buffer EB
15 ml
20 ml
DNA Spin Column
100 pcs
200 pcs
Collection Tube£¨2ml£©
100 pcs
200 pcs

All reagents are stable for 24 months at RT, when stored properly.
1.  If RNaseA is inactive, RNA will contaminate the plasmid. Add additional RNaseA to Buffer P1.
2.  Check Buffer P2 for SDS precipitation due to low storage temperatures. If necessary, dissolve the SDS by warming to 37¡ãC
3.  Please ensure the bottles of buffer tightly capped when not in use to prevent reagents evaporating, oxidation and pH changing.
4.  Dilute Buffer WB with three volume absolute ethanol before start.
(1)Rapid and convenient. Do not contain poisonous phenol etc and not need carrying ethanol precipitation. Multi-elution can ensure high-purified DNA, which can be applied to all kinds of molecular experiments such as PCR£¬Southern-blot and digestions directly.
(2)The silica membranes in the spin-column come from the world-famous company.
(3)Unique content can effectively remove the nuclease, even apply to rich-nuclease stains of JM and HB101,
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Guangzhou Geneshun Biotech Ltd ÔÁICP±¸08201538ºÅ
Tel£º020-23312160 Fax£º020-87056567