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Super M-MuLV Reverse Transcriptase
Super M-MuLV Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA- and DNA-dependent polymerase activity, but lacks ribonuclease H activity specific to RNA in RNA-DNA hybrids . RNase H activity is eliminated by a point mutation in the RNase H domain of M-MuLV RT.
it can synthesize long DNA chains.
Product component:
Super M-MuLV Reverse Transcriptase200U/µl
5X Reaction Buffer
1 ml5
Store at C20C   
Recombinant E.coli contains Moloney murine leukemia virus reverse transcriptase gene from clone of Moloney murine.
Efficient synthesis of first strand cDNA up to 12 kb.
Inhibition and Inactivation:
Inhibitors: metal chelators, inorganic phosphate, pyrophosphate and polyamines .
Inactivated by heating at 70 for 5 min.
First strand cDNA synthesis.
RT-PCR and real-time RT-PCR.
Synthesis of cDNA for PCR, cloning, and hybridization probes.
Generation of labeled cDNA probes for micro arrays.
DNA labeling.
Analysis of RNA by primer extension.
Unit Definition:
One unit is that amount of enzyme required to incorporate 1 nmol of deoxynucleotide into DE-81 filter-binding material in 10 min at 37C using poly (rA)-oligo(dT)12 - 18 as template-primer
Assay Conditions:
The reaction mixture (25 µl) contains 50mM Tris-HCI (pH 8.3), 40mM KCl, 6mM MgCl2, 1mM DTT, 400µM poly (rA)-oligo(dT)12 - 18, 500µM radiolabeled TTP and 0.1 - 0.5 units enzyme. Incubation is at 37C for 10 min.
Storage Buffer:
20mM Tris-HCl (pH 7.5), 0.1M NaCl, 0.1mM EDTA, 5mM DTT, 0.1% Triton X-100 and 50% glycerol.
5X Reaction Buffer:
250mM Tris-HCl (pH 8.3 at 25C), 250mM KCl, 20mM MgCl2, 50mM DTT.
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